Journal: Evidence-based Complementary and Alternative Medicine : eCAM
Article Title: Gene Expression Profiles Underlying Selective T-Cell-Mediated Immunity Activity of a Chinese Medicine Granule on Mice Infected with Influenza Virus H1N1
doi: 10.1155/2014/976364
Figure Lengend Snippet: Genes associated with effector T cells activation and cytokines involved in T-cell-mediated immunity.
Article Snippet: Membranes were incubated overnight and probed with rabbit anti-mouse IL-4 (bs0581R) and IFN- γ (bs0480R) polyclonal antibody (Biosynthesis Biotechnology Co., LTD, Beijing), rabbit anti-mouse TNF- α (BC0088) and IL-1 β (BA2782) polyclonal antibody (Boster Biological Co., LTD, WuHan), rabbit anti-human JNK (4764S) and p38 (9252S) polyclonal antibody (Cell Signaling Technology, Inc), rabbit anti-mouse MyD88 (bs1047R) monoclonal antibody (Biosynthesis Biotechnology Co., LTD, Beijing), rabbit anti-human TLR7 (bs6601R) polyclonal antibody (Biosynthesis Biotechnology Co., LTD, Beijing), and rabbit anti-GAPDH (Sigma, USA).
Techniques: Activation Assay